Enzyme recovery from waste activated sludge

Proceedings

Enzyme recovery from waste activated sludge

Smith, S.R. and Liu, Z., Imperial College London, UK

(free)

Enzymes are high value industrial bio-catalysts with extensive applications in a wide range of
manufacturing and processing sectors. However, the culture medium for enzyme production is a major
reason for the high cost of industrial enzymes. Wastewater treatment and generated biological sludges
provide an alternative source of enzymes to conventional industrial production methods. Here, we
present a robust extraction protocol for recovering enzymes from activated sludge using ultrasonication
and surfactant treatment. Under optimum conditions (40 % amplitude, 10 min duration), ultrasound
disruption of activated sludge gave recovery rates of protease and cellulase enzymes equivalent to 63.1
% and ~100 %, respectively. The recovery of enzymes from activated sludge represents a potentially
significant, high value, resource recovery option for biological sludge generated by municipal
wastewater treatment.

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